25% (n = 10) were diagnosed as BMD and the remaining one case (3.12%) is Nine of the 32 patients were females. It shows that IHC can also be performed on formalin fixed paraffin embedded muscle tissues. In this study comprising of 23 males and 9 females, we used paraffin blocks of skeletal muscle tissue and performed IHC using dystrophin and beta-spectrin antibodies in the diagnosis of various types of muscular dystrophies. In Pakistan diagnosis of muscular dystrophies is still dependent on clinical features and creatine phosphokinase
(CPK) values. This is the first study from the subcontinent performing GW786034 concentration immunohistochemistry successfully on formaline fixed paraffin embedded (FFPE) blocks for the diagnosis of muscular dystrophies.”
“Proteins belonging to the serine protease inhibitor (serpin) superfamily play essential roles in many organisms. find more In arthropods these proteins are involved in innate immune system, morphogenesis and development. In mammals serpins regulate pathways that are essential to life such as blood coagulation, fibrinolysis, inflammation
and complement activation, some of which are considered the host’s first line of defense to hematophagous and/or blood dueling parasites. Thus, it is hypothesized that ticks use serpins to evade host defense, facilitating parasitism. This study describes eighteen full-length cDNA sequences encoding serpins identified in Rhipicephalus (Boophilus) microplus, here named RmS 1-18 (R. microplus serpin). Spatial and temporal transcriptional profiling demonstrated that R. microplus serpins are transcribed during feeding, suggesting their participation in tick learn more physiology regulation. We speculate that the majority of R. microplus serpins are conserved in other ticks, as indicated by phylogeny analysis. Over half of the 18 RmSs are putatively functional in the extracellular environment, as indicated by putative signal peptides on 11 of 18 serpins. Comparative modeling and structural-based alignment
revealed that R. microplus serpins in this study retain the consensus secondary of typical serpins. This descriptive study enlarges the knowledge on the molecular biology of R. microplus, an important tick species. (C) 2013 Elsevier Inc. All rights reserved.”
“Background Allergic contact dermatitis is a leading cause of periorbital dermatitis. The extremely thin nature of the periorbital skin may facilitate allergen penetration, making this area particularly susceptible to allergic contact sensitisation. In this study, we assessed sensitisation rates to ingredients of common topical ophthalmic agents. Materials and methods Data collected by 57 participating centres of the Information Network of Departments of Dermatology (IVDK) were analysed retrospectively. Of the 101 403 patients patch tested between January 2001 and December 2010, 4779 patients suffered from periorbital dermatitis and 1158 patients were specifically tested to the ophthalmic tray.
The conjugate addition reactions proceeded smoothly in the presence of 5 mol % of the chiral strontium catalyst, at room temperature, to afford the desired find more adducts in high yields and excellent enantioselectivities. This method provides an. efficient approach to the preparation of building blocks possessing various functional groups and possible sites for further functionalization.”
There are numerous challenges to successfully integrating palliative care in the intensive care unit. Our primary goal was to describe and compare the quality of palliative care delivered in an intensive care unit as rated by physicians and nurses working in that intensive care unit.\n\nDesign: Multisite study using self-report questionnaires.\n\nSetting: Thirteen hospitals throughout the United States.\n\nParticipants: Convenience
sample of 188 physicians working in critical care (attending physicians, critical care fellows, resident physicians) and 289 critical care nurses.\n\nMeasurements and Main Results: Clinicians provided overall ratings of the care delivered by either nurses or physicians in their intensive care unit for each of seven domains EPZ-6438 Epigenetics inhibitor of intensive care unit palliative care using a 0-10 scale (0 indicating the worst possible and 10 indicating the best possible care). Analyses included descriptive statistics to characterize measurement characteristics of the ten items, paired Wilcoxon tests comparing item ratings for the domain of symptom management with all other item ratings, and regression analyses assessing differences in ratings within and between clinical disciplines. We used p < .001 to denote statistical significance to address multiple comparisons. The ten items demonstrated good content validity with few missing responses or ceiling or floor effects. Items receiving the lowest ratings assessed spiritual
support for families, emotional support for intensive care unit clinicians, and palliative-care education for intensive care unit clinicians. All but two items were rated significantly lower than the item assessing symptom management (p < .001). Nurses rated nursing BI 2536 clinical trial care significantly higher (p < .001) than physicians rated physician care in five domains. In addition, although nurses and physicians gave comparable ratings to palliative care delivered by nurses, nurses’ and physicians’ ratings of physician care were significantly different with nurse ratings of this care lower than physician ratings on all but one domain.\n\nConclusion: Our study supports the content validity of the ten overall rating items and supports the need for improvement in several aspects of palliative care, including spiritual support for families, emotional support for clinicians, and clinician education about palliative care in the intensive care unit. Furthermore, our findings provide some preliminary support for surveying intensive care unit clinicians as one way to assess the quality of palliative care in the intensive care unit.
“Two experiments were conducted to assess the effect of the reduction of the crude protein (CP) content of diets supplemented with amino acids on piglets weighing 6-15
kg. In the performance experiment (Experiment I), 120 piglets weaned at 21 days of age with initial live weights of 5.95 +/- 0.33 kg were distributed into five treatment groups. This grouping followed a randomized block design with eight repetitions and three animals per experimental unit. The treatments consisted of five different diets, in which the CP content were reduced from 21.0% to 15.0% (21.0%, 19.5%, 18.0%, 16.5%, and MK-1775 inhibitor 15.0% CP); the amino acid requirements of the diet were met by adding L-lysine, DL-methionine, L-threonine, L-tryptophan, L-valine, and L-isoleucine. No differences were found in the variables associated with performance among animals from different treatment groups. Therefore, any of the investigated CP levels can effectively be used in piglet diets supplemented with synthetic amino acids. The essential/nonessential amino
acid ratio (EAA:NEAA) increased with the reduction of PD-1/PD-L1 Inhibitor 3 mouse the CP content, and the best ratio (53:47) was achieved with the diet containing 15% protein. Urea concentrations decreased linearly with protein reduction (Experiment I). To assess the nitrogen balance (Experiment II), 20 crossbred male castrated piglets from a commercial lineage, weaned at 21 days of age, were randomly assigned in two blocks, in which each block had two replicates
(four replicates per treatment). The average live weight of the piglets was 10.79 +/- 2.19 kg. The animals were housed in metal cages and were distributed into five treatment groups following a randomized block design with four repetitions; the experimental unit consisted of one piglet. The nitrogen excretion and blood and urine urea concentrations decreased linearly (P smaller than 0.05) with the reduction of CP in the diets, resulting in reduced nitrogen excretion into the environment. (C) 2014 Elsevier B.V. All rights reserved.”
“Intromugil alachuaensis n. sp. is described based on specimens collected from the flathead grey mullet (Mugil cephalus) from the Santa Fe River in Florida. The new species is the KPT-8602 inhibitor fourth recognized species in the genus and the second from North America, with the other 2 being confined to South America. Intromugil mugilicolus from Louisiana and Mississippi is redescribed based on the holotype and newly collected material that was not flattened prior to fixation. Two generic features not previously reported are apparent in the new material from I. mugilicolus and I. alachuaensis n. sp.: an armed oral sucker and a series of sacs containing glandular material arranged in symmetrical rows in the hermaphroditic duct. Intromugil alachuaensis differs from I. mugilicolus by having an oral sucker longer than wide, body spines smaller and lanceolate rather than longer and hastate, and smaller vitelline follicles. Intromugil alachuaensis n. sp.
In layer 1 of this approach, efficacy and safety of a study drug are evaluated through the overall study results; layer 2 entails evaluation of whether there is inconsistency in efficacy and/or safety of the study drug for a specific subgroup with overall results; and in layer 3, the results of layers 1 and 2 are used to evaluate benefits and risks in each applying country. The 3-layer approach can be used to create
a globally common model using data collected in all countries Selleck MGCD0103 in the study. This global evaluation allows benefits and risks to be evaluated in all countries and should allow globalized CTDs to be developed. Alignment between research and development sites by pharmaceutical companies and success of regulatory conventions can reduce the total amount of review time. Ultimately, these changes would lead to faster approval of new drugs.”
“In mammals, check details the trophoblast lineage of the embryo is specified before attachment/implantation to become the fetal portion of the placenta. Trophoblast-derived cells were isolated and cultured from day 10 and day 13 porcine embryos and were grown in vitro in a defined, serum-free culture medium for over 2 years without showing any signs of senescence.
However, trophoblast-derived cells placed into serum-containing medium rapidly senesce and fail to proliferate. Semiquantitative and quantitative gene expression analyses of cells in culture from 0 to 30 days BMS-777607 supplier confirmed the presence (and relative abundance) of mRNA transcripts from genes involved in trophoblast function (CDX2, TEAD4, CYP17A1, HSD17B1, FGFR2, PLET, HAND 1) as well as some genes known to mediate pluripotency (POU5F1, KLF4, CMYC). Protein immunolocalization demonstrated expression of both trophoblast and mesenchymal cell markers. DNA methylation patterns in promoters of three critical developmental genes (HAND 1, KLF4, TEAD4) did not change appreciably over 4 months of culture in vitro. It was demonstrated that these trophoblast-derived cells are easily stably transfected with an
exogenous transgene (eGFP) by a variety of methods, and show the ability to survive and to be passaged repeatedly after transfection. In summary, early embryonic porcine trophoblast-derived cells have demonstrated unique characteristics, which means they could be used as valuable tools for laboratory work. Anticipated applications include the study of trophoblast physiology as well as possible solutions for improving efficiency of transgenesis by somatic cell nuclear transfer and for pluripotency reprogramming of cells. Published by Elsevier B.V.”
“Ambalavanan N, Stanishevsky A, Bulger A, Halloran B, Steele C, Vohra Y, Matalon S. Titanium oxide nanoparticle instillation induces inflammation and inhibits lung development in mice. Am J Physiol Lung Cell Mol Physiol 304: L152-L161, 2013. First published December 7, 2012; doi: 10.1152/ajplung.00013.2012.
The present study aimed to elucidate the effect of COX-2 inhibitors on the pilocarpine-induced epileptic status rats and the possible mechanism of action. Celecoxib was administered 45 min prior to the pilocarpine
administration. Celecoxib attenuated the likelihood of developing spontaneous recurrent seizures after pilocarpine-induced prolonged seizure. COX-2 protein expression was increased in hippocampus, which peaked 1 h and increased persistently 28 days after seizures. During the latent period, Celecoxib GW786034 purchase prevented microglia activation in the hilus and inhibited the abnormal neurogenesis and astrogliosis in the hippocampus. Celecoxib attenuated the activation of mitogen-activated protein kinase (MAPK). Phosphorylation of extracellular signal-regulated protein kinase (ERK) expression in the epilepsy-only group was 1.4 and 1.2 times higher than that of the epilepsy-celecoxib group at 1 and 4 days after status epilepticus (SE), respectively. MAPK/ERK is a signal pathway related to cell proliferation and differentiation. Protein expression from C-fos, an immediate early gene, was downregulated after celecoxib treatment. Also celecoxib upregulates the expression of GABA(A) receptors, mediating the majority of fast inhibitor synaptic transmissions in the brain. NS-398, another
cox-2 inhibitor, enhanced the frequency and decay time of miniature inhibited PLX4032 supplier postsynaptic currents (mIPSCs). In conclusion, the results of the present study strongly suggest the possible role of COX-2 in the pathophysiology of epilepsy and that the use of COX-2 inhibitors might be an adjuvant therapy in the treatment of MK0683 epilepsy.”
“Objective: To assess the rate
of recurrence and complications after primary pterygium removal with the P.E.R.F.E.C.T. for PTERYGIUM (pterygium extended removal followed by extended conjunctival transplantation) technique.\n\nDesign: A case series study of the P.E.R.F.E.C.T. for PTERYGIUM technique was conducted by 1 surgeon with a 1-year follow-up to assess the recurrence and complication rate.\n\nParticipants: Two hundred fifty consecutive primary pterygium removals.\n\nIntervention: A major modification of conjunctival autograft surgery was used to treat primary pterygia. Main Outcome Measures: The recurrence rate and complications after excision of primary pterygia using the P.E.R.F.E.C.T. for PTERYGIUM technique.\n\nResults: One patient had a vascularized delle listed as a recurrence from among 250 consecutive patients (0.4%). The mean study follow-up period was 462 +/- 172 days. No patient lost best-corrected vision.\n\nConclusions: The P.E.R.F.E.C.T. for PTERYGIUM technique results in a near 0% recurrence rate with minimal complications and a good cosmetic appearance.”
“The goal of this work was to examine the possible influence of periclavicular irradiation on outcome of breast cancer patients with 1-3 positive lymph nodes with special emphasis on late toxicity rates.
(C) 2010 Elsevier B.V. All rights reserved.”
“Background. The lack of knowledge about epidemiological and clinical data of patients with Acquired Brain Injury (ABI) admitted to Rehabilitation facilities in Italy led to the creation, in June selleckchem 2008, of a data collection on-line registry.\n\nAim. To collect epidemiological and clinical data and to evaluate functional outcome of patients with severe traumatic and non-traumatic ABI admitted to Rehabilitation
facilities in Italy between June 2008 and December 2011 and to compare data of patients with ABI of different aetiologies.\n\nDesign. Observational retrospective study.\n\nSetting. The study involved 29 Italian Rehabilitation facilities.\n\nPopulation. The study enrolled 1469 patients with severe traumatic (TBI) and non-traumatic ABI (NTBI).\n\nMethods. Data collected included demographic (number of patients with TBI and NTBI, gender, age) and clinical characteristics (provenience, number of days elapsed between onset and rehabilitation admission, rehabilitation length of stay, discharge destination, death
and vegetative state diagnosis, presence of percutaneous endoscopic gastrostomy, tracheostomy, Rabusertib price pressure sores and paraosteoarthropathies). Functional outcome was evaluated using the Disability Rating Scale.\n\nResults. Of the whole population studied, 44.31% and 55.69% patients had suffered a TBI and a NTBI, respectively. In the NTBI group 40.09% had a cerebrovascular injury, 12.04% an anoxic brain damage, 3.6% had a brain injury of other causes. The mean age was 43.67 and 56.68 for subjects with TBI and NTBI, respectively. Patients with TBI showed a lower onset-admission interval (OAI), compared with NTBI group; no difference in rehabilitation length of stay (LOS) was recorded between groups. Patients with TBI presented
a lower DRS score at admission and discharge and returned home more frequently than NTBI Proteasome inhibition assay group.\n\nConclusions. The creation of a National registry allows the collection of data about patients with ABI in order to study the clinical course, the functional outcome and to establish a basis for comparison with other data sources.\n\nClinical Rehabilitation Impact. Data collection could be useful in the evaluation and planning of rehabilitation pathways, and to assess the allocation of healthcare and rehabilitative resources.”
“Recently, a new recipe for developing and deploying real-time systems has become increasingly adopted in the JET tokamak. Powered by the advent of x86 multi-core technology and the reliability of JET’s well established Real-Time Data Network (RTDN) to handle all real-time I/O, an official Linux vanilla kernel has been demonstrated to be able to provide real-time performance to user-space applications that are required to meet stringent timing constraints.
(ClinicalTrials. gov number, NCT00132691.) (Invest Ophthalmol Vis Sci. 2012;53:1169-1176) DOI:10.1167/iovs.11-8259″
“Colonic diverticular bleeding can usually be managed with conservative treatment. However, in a selected group of patients
under conditions of recurrent, www.selleckchem.com/products/ferrostatin-1-fer-1.html persistent bleeding influencing quality of life or causing life-threatening shock, it should be managed with surgery. This is a retrospective study to clarify the risk factors relating to colectomy for colonic diverticular bleeding.\n\nBetween 1997 and 2005, a retrospective chart review of 73 patients with colonic diverticular bleeding was undertaken. Univariate and multivariate logistic regression analyses were performed to identify the relevant risk factors correlating to colectomy.\n\nThe mean age of the 73 patients was 70
years (range, 22-90 years). Most colonic diverticular bleeding could be managed with conservative treatment (n = 63, 86.3%), and urgent colectomy was performed in ten patients (13.7%). The bleeding site could not be well identified in six of those ten patients and so underwent total abdominal colectomy with ileorectal anastomosis, and the other four underwent right hemicolectomy after a diagnosis JQ-EZ-05 of right-sided colon diverticula with bleeding. There were two deaths in the surgical group and one death in the nonsurgical group. The overall mortality rate in the series was 4.11% and 20% among patients undergoing urgent colectomy. Multiple logistic regression analysis showed
Selleck S63845 that the presence of comorbidities and daily maximum blood transfusion requirement were risk factors for urgent colectomy for colonic diverticular bleeding.\n\nPreoperative comorbid diseases may increase operative risk in urgent surgery, and the outcome is poor. To avoid high mortality and morbidity relating to the urgent colectomy, we suggest that patients of colonic diverticular bleeding with comorbid diseases, especially subgroups of patients with diabetes and gouty arthritis, may need early elective colectomy.”
“Purpose: Postoperative ileus (POI) is an impairment of coordinated gastrointestinal (GI) motility that develops as a consequence of abdominal surgery and is a major factor contributing to patient morbidity and prolonged hospitalization. The aim of this study was to investigate the effects of different 5-hydroxytryptamine 4 (5-HT4) receptor agonists, which stimulate excitatory pathways, on a POI model. Materials and Methods: The experimental model of POI in guinea pigs was created by laparotomy, gentle manipulation of the cecum for 60 seconds, and closure by suture, all under anesthesia. Different degrees of restoration of GI transit were measured by the migration of charcoal.
Behavioral indices of alcohol-induced premature responding correlated with the current
drinking levels and impulsivity traits, suggesting an interaction between alcohol effects and personality predispositions. A distributed frontoparietal cortical network was activated by incongruity. However, moderate alcohol inebriation selectively attenuated anterior cingulate cortex (ACC) activation during both high-conflict trials and erroneous responses, indicating vulnerability of the regulative function subserved by the ACC. By disrupting topdown, strategic processing, alcohol may interfere with goal-directed behavior, resulting in poor self control. The present results support G418 solubility dmso models proposing that alcohol-induced prefrontal impairments diminish inhibitory control and are modulated by dispositional risk factors and levels of alcohol consumption. Hum Brain Mapp, 2012. (C) 2011 Wiley Periodicals, Inc.”
“Background: Pulmonary arterial hypertension is characterized by increased thickness of pulmonary vessel walls due to both increased proliferation of pulmonary arterial smooth muscle cell (PASMC) and deposition of extracellular matrix. In patients suffering
from pulmonary arterial hypertension, endothelin-1 AZD3965 (ET-1) synthesis is up-regulated and may increase PASMC activity and vessel wall remodeling through transforming growth factor beta-1 (TGF-beta 1) and connective tissue growth factor.\n\nObjective: To assess the signaling pathway leading to ET-1 induced proliferation and extracellular matrix deposition by human PASMC.\n\nMethods: PASMC were serum starved for 24 hours before stimulation with either ET-1 and/or TGF-beta 1. ET-1 was inhibited by Bosentan, ERK1/2 mitogen activated protein kinase (MAPK) was inhibited by U0126 and p38 MAPK was inhibited by Selleckchem Compound C SB203580.\n\nResults: ET-1 increased PASMC proliferation when combined with serum. This effect involved the mitogen activated protein kinases (MAPK) ERK1/2 MAPK and was abrogated by Bosentan which caused a G1- arrest through
activation of p27((Kip)). Regarding the contribution of extracellular matrix deposition in vessel wall remodeling, TGF-beta 1 increased the deposition of collagen type-I and fibronectin, which was further increased when ET-1 was added mainly through ERK1/2 MAPK. In contrast, collagen type-IV was not affected by ET-1. Bosentan dose-dependently reduced the stimulatory effect of ET-1 on collagen type-I and fibronectin, but had no effect on TGF-beta 1.\n\nConclusion and Clinical Relevance: ET-1 alone does not induce PASMC proliferation and extracellular matrix deposition. However, ET-1 significantly up-regulates serum induced proliferation and TGF-beta 1 induced extracellular matrix deposition, specifically of collagen type-I and fibronectin. The synergistic effects of ET-1 on serum and TGF-beta 1 involve ERK1/2 MAPK and may thus present a novel mode of action in the pathogenesis of pulmonary arterial hypertension.
Slowing down the heavier trailing sound limb, compared to the prosthetic limb, results in a relatively larger braking force at the end of the swing phase. The simulations showed that only narrow ranges of leading limb angle and ankle moments could be used to achieve the same CoM velocities with the mathematical
https://www.selleckchem.com/products/beta-nicotinamide-mononucleotide.html model as the average start and end velocities of the prosthetic limb user. We conclude that users of prosthetic limbs have a narrow range of options for the dynamics variables to achieve a target CoM velocity. The lack of active control in the passive prosthetic ankle prevents the TF amputee subjects from producing sufficient braking force when terminating gait with the prosthetic limb leading, forcing the subjects to use both limbs as a functional unit, in which the sound limb is mostly responsible for the gait termination. (C) 2012 IPEM. Published by Elsevier Ltd. All rights reserved.”
“Fluorescence resonance energy transfer (FRET) microscopy is a powerful technique to quantify dynamic protein-protein interactions in live cells. Total internal reflection fluorescence
(TIRF) microscopy can selectively excite molecules within about 150 nm of the glass-cell interface. Recently, these two approaches were combined to enable high-resolution FRET imaging on the adherent surface of living cells. Here, we show that interference fringing Nutlin-3a inhibitor of the coherent laser excitation used in TIRF creates lateral heterogeneities that impair quantitative TIRF-FRET measurements. We overcome this limitation by using a two-dimensional scan head to rotate laser beams for donor and acceptor excitation around
the back focal plane of a high numerical aperture objective. By setting different radii for the circles traced out by each laser in the back focal plane, the penetration depth was corrected for different wavelengths. These buy Ion Channel Ligand Library modifications quell spatial variations in illumination and permit calibration for quantitative TIRF-FRET microscopy. The capability of TIRF-FRET was demonstrated by imaging assembled cyan and yellow fluorescent protein-tagged HIV-Gag molecules in single virions on the surfaces of living cells. These interactions are shown to be distinct from crowding of HIV-Gag in lipid rafts.”
“Objective: To study the treatment patterns and visual outcome over one year in Asian patients with choroidal neovascular membrane secondary to age-related macular degeneration (AMD-CNV) and polypoidal choroidal vasculopathy (PCV). Design: Prospective cohort, non-interventional study. Methods: 132 treatment-naive patients who received treatment for AMD-CNV and PCV were included. All patients underwent standardized examination procedures including retinal imaging at baseline and follow-up. AMD-CNV and PCV were defined on fundus fluorescein angiography and indocyanine green angiography at baseline.
The mechanisms involved in long-distance trafficking of FT/FTL2 remain to be elucidated. In this study, we identified the critical motifs on both FT and FTL2 required for cell-to-cell trafficking through mutant analyses using a zucchini yellow mosaic virus expression vector. Western blot analysis, performed on phloem sap collected from just beneath the vegetative apex of C.moschata plants, established that all mutant proteins tested retained the ability to enter the phloem translocation stream. However, immunolocalization RSL-3 studies revealed that a number of these FTL2/FT mutants were defective
in the post-phloem zone, suggesting that a regulation mechanism for FT trafficking exists in the post-phloem unloading step. The selective movements of FT/FTL2 were further observed by microinjection and trichome rescue studies, which revealed that FT/FTL2 has the ability to dilate
plasmodesmata microchannels during the process of cell-to-cell trafficking, and various mutants were compromised in their capacity to traffic through plasmodesmata. Based on these findings, a model is presented to account for the mechanism by which FT/FTL2 enters the phloem translocation stream and subsequently exits the phloem and enters the apical tissue, where it initiates the vegetative to floral transition.”
“Antibiotic use in the treatment of respiratory MEK162 order tract infections is common in primary care. The European Surveillance of Antimicrobial Consumption (ESAC programme), collecting data from 35 countries, showed that antibiotic use was highest in southern European countries. Increased antibiotic consumption has been shown by numerous ecological studies Anlotinib in vivo to contribute to the emergence of antibiotic resistance in streptococci. A study comparing outpatient antibiotic consumption in the USA showed it to be similar to that in southern European countries, but macrolides, particularly azithromycin, are among the first-line agents prescribed in the USA for respiratory infections. In Europe, patients are more likely to receive a beta-lactam;
and when a macrolide is indicated, clarithromycin is more likely to be prescribed than azithromycin. Streptococci resistance to macrolides can be acquired via two mechanisms: by the mef gene, which encodes for the efflux pump mechanism, producing low to moderate resistance, or the erm gene (post-transcriptional modification of the bacterial ribosomal unit), resulting in high resistance. Macrolide resistance is mediated by erm(B) and mef(A) alone or in combination. A surveillance study showed that mef was responsible for most of the macrolide resistance seen in the USA; a decrease in the number of isolates carrying mef(A) was associated with a doubling of the number of isolates carrying both mef(A) and erm(B).