In the 3-deazaneplanocin A other two categories, score 0 and 3+, the agreement was substantial /almost perfect (greater than 0.80). Table 3 k cs statistic and 95% Jackknife confidence interval by HER2 score Score N slides kcs 95% Confidence

interval of kcs       Lower limit Upper limit 0 64 0.80 0.64 0.97 1+ 64 0.54 0.31 0.78 2+ 64 0.37 0.07 0.70 3+ 64 0.85 0.70 1.00 EQA HER2 interpretation Table 4 summarizes the results obtained from the EQA HER2 interpretation step. Only two PCs provided scores equal to reference ones for all the 10 slides. Four PCs provided one discordant value out of 10, misclassifying the reference value score 1+ in three cases and score 2+ in one case. It is worthy to note, that

no score 3+ was misclassified and only 1 score 0 was interpreted as score 1+. Conversely, we observed 12 and 14 misclassifications in score 1+ and 2+, respectively. Table 4 HER2 interpretation: misclassifications in relation to the reference score ID Group Total N° of misclassified slides(#) Reference score 0(#) Reference score 1 + (#) Reference BIBW2992 score 2 + (#) Reference score 3 + (#) PC1 3 1/10 0/2 1/3 [2+] 0/3 0/2 PC2 3 2/10 0/2 0/3 2/3 [1+;1+] 0/2 PC3 1 1/10 0/2 1/3 (*) 0/3 0/2 PC4 1 2/10 0/2 0/3 2/3 [1+;1+] 0/2 PC5 3 0/10 0/2 0/3 0/3 0/2 PC6 2 2/10 0/2 1/3 [2+] 1/3 [3+] 0/2 PC7 3 0/10 0/2 0/3 0/3 0/2 PC8 1 2/10 1/2 [1+] ^ 0/3 1/3 Thymidine kinase [1+] 0/2 PC9 2 1/10 0/2 1/3 [2+] 0/3 0/2 PC10 2 2/10 0/2 1/3 [2+] 1/3 [1+] 0/2 PC11 2 2/10 0/2 1/3

[2+] 1/3 [1+] 0/2 PC12 1 2/10 0/2 1/3 [2+] 1/3 [1+] 0/2 PC13 3 3/10 0/2 2/3 [2+;2+] 1/3 [1+] 0/2 PC14 1 1/10 0/2 0/3 1/3 [1+] 0/2 PC15 2 2/10 0/2 1/3 [2+] 1/3 [3+] 0/2 PC16 3 4/10 0/2 2/3 [0;0] 2/3 [1+;1+] 0/2 Total 27/160 1/32 12/48 14/48 0/32   (*) Slide not evaluated. (#)N° of misclassified slides/N° of received slides. ^Brackets report the score provided by PCs. Table 5 shows the kw values and the relative lower limit of the 95% confidence interval obtained by comparing the scores provided by PCs with the reference values. Overall, by considering the point-estimate values of the kw statistic a satisfactory agreement was reached between the reference score and the one provided by the evaluation of each PC. However, by taking into account the lower PLX4032 limits of the 95% confidence interval of the kw statistic, only 6 PCs reach a fully satisfactory agreement. Table 5 k w statistic values between the reference scores and scores reported by PCs ID Group kw kw 95% CI       Lower limit Upper limit PC1* 3 0.95 0.86 1.00 PC2 3 0.90 0.76 1.00 PC3* 1 1.00 1.00 1.00 PC4 1 0.90 0.76 1.00 PC5* 3 1.00 1.00 1.00 PC6 2 0.91 0.80 1.00 PC7* 3 1.00 1.00 1.00 PC8 1 0.89 0.72 1.00 PC9* 2 0.95 0.86 1.00 PC10 2 0.90 0.77 1.