The number of cycles was 35. The changes in gene expression (n-fold) selleck compound calculated from the qRT-PCR data. Analysis of relative gene expression data was done using the 2-2∆∆CT method
as described previously [41]. The 16S rRNA was used as the internal controls. Statistical analysis All experiments were repeated a minimum of three times, and data are expressed as mean ± SD. Differences were considered significant for P < 0.05 (*, P value 0.05-0.01; **, P value <0.01). Comparison of two groups was made with an unpaired, two-tailed student’s t-test. Comparison of multiple groups was made with ANOVA. Acknowledgements The study was not supported by any external funding. References 1. Silva J, Leite D, Fernandes M, Mena C, Gibbs PA, Teixeira P: Campylobacter spp. as a Foodborne Pathogen: a review. Front Microbiol 2011, 2:1–12. article number 200 2. Olson CK, Ethelberg S, van Pelt W, Tauxe RV: Epidemiology of Campylobacter jejuni infections in industrialized nations. In Campylobacter. Edited by: Nachamkin I, Szymanski C, Blaser MJ. Washigton,
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