The development of apple and tomato fruit, from anthesis http://www.selleckchem.com/products/SB-203580.html to mature fruit differs in length, however we compared patterns of expression during similar Inhibitors,Modulators,Libraries phases of develop ment, in particular the mid development phase when cells are expanding in both apple and tomato. Of the 47 genes for which expression patterns were compared, 16 had similar patterns of expression in both apple and tomato and are shown in Figure 7, with the cell expansion and ripening stages highlighted. A fur ther five genes had some similarity of expression but 26 had little or no similarity of expression. For genes such as Tubulin, SAM syn thase and an expansin homologue more than one tomato sequence had homology to an apple gene and in the case of the tubulin genes to three apple genes.

Inhibitors,Modulators,Libraries For the tubulin genes the pat terns of expression mostly differed between apple and tomato but one of the tomato genes showed a steady decrease in expression during cell expansion similar to the apple genes. For the three tomato SAM synthase genes only one had a pattern of expression similar to the apple gene suggesting this tomato gene may have a similar function in apple and tomato. For the two tomato expansin homologues with similarity to apple, SGN U312953 increased in Inhibitors,Modulators,Libraries expression during ripening whereas SGN U333609 and the apple expansin homo logue Inhibitors,Modulators,Libraries both increased during cell expansion and declined in ripening, suggesting these genes may be orthologues and have a role during cell enlargement but not in fruit softening. Four genes without annotation were identified as having similar patterns of expression in apple and tomato fruit.

Further bioinformatic analysis suggests that, Comparison Inhibitors,Modulators,Libraries of gene expression between apple cultivars A recent report has examined expression of apple genes early in fruit development using an array of 3484 cDNAs. These authors identified 88 unique apple genes Ceritinib expressed more in whole young fruit than in whole mature fruit in the cultivar Fuji. Eighty four homologues of these genes were identified in our EST database, 42 of these were represented on our microarray. Of these 42 genes, 17 were selected as changing signifi cantly during fruit development, 13 in the EFD cluster and four in the MD cluster. Since the criteria used to select significantly changing genes was fairly stringent we plotted expression patterns for all the matches between our data and the selected early fruit development genes from Lee et al. in order to identify any additional genes with similar patterns of expression. Eighteen genes identified by Lee et al. as being up regulated were not confirmed in our microarray, however, an additional 13 genes were identified with high expression early in Royal Gala fruit development, and low expression in ripening.