The C4 compound was effective in reducing the lipid peroxidation

The C4 compound was effective in reducing the lipid peroxidation at the lowest concentration FG-4592 in vitro tested. The IC50 values of the compounds followed the order: C4 < C2 < C3 < C1 against Fe(II)-induced lipid peroxidation (Table 2). For SNP-induced lipid peroxidation, the IC50 values of the compounds followed the order: C4 < C3 < C2 < C1 (Table 2). The Imax values of the compounds against Fe(II)-induced lipid peroxidation was 67%, 81%, 72% and 90% respectively of C1 to C4 ( Table 4). For SNP-induced

lipid peroxidation, the Imax values of the compounds was 69%, 79%, 89% and 93% respectively of C1 to C4 ( Table 4). The organoselenium compounds did not show any significant effects in tests involving Fe(II)-chelating properties, free radical scavenging, thiol-oxidase activities and cellular viability

(data not shown). The curve of ascorbic acid was determine utilizing the concentration 5, 10, 20, 40 and 80 μM represented at Fig. 4 as the letters a–e. The diselenides at 400 μM showed total antioxidant activity similar Trametinib chemical structure to ascorbic acid at 10, 20 and 40 μM. Similarly, the monoselenides at 400 μM demonstrated an antioxidant effect equivalent to that of ascorbic acid at 5, 10 and 20 μM. Fig. 5 demonstrates the GPx activity of the organoselenium compounds. The compounds C1 (Fig. 5A) and C2 (Fig. 5B) did not present any significant GPx activity when compared with the control group. DMSO alone had no significant effect on the GPx activity. However, our data reveals that DPDS, C3 (Fig. 5C) and C4 analogs (Fig. 5D) at both concentrations tested demonstrated GPx-like activity. The monoselenides did not show TrxR activity, while the diselenides demonstrated a significant difference compared to the control

group. As shown in Fig. 6, C3 and C4 demonstrated 13 and 7 times higher TrxR activity, respectively, than the control. The present study aimed to investigate and clarify the antioxidant properties of novel mono- and diselenides compounds. Oxidative stress is involved in various metabolic disorders and in the normal process of aging (Giles et al., 2012 and Mugesh et al., 2001). Additionally, antioxidant therapy has been used in an attempt to repair these harmful effects (Nogueira and Rocha, 2011 and Zadra et al., 2012). In this context, lipid G protein-coupled receptor kinase peroxidation products MDA and 4-hydroxynonenal have been shown to play significant roles in brain and liver toxicities and can serve as markers of oxidative damage (Chen et al., 2005). Prestes reported that monoselenides, which possess an amino group near the selenium, exhibited decreased MDA formation compared to that found for DPDS (Prestes et al., 2012). The novel mono- and diselenides compounds examined in our study demonstrated antioxidant activity against Fe (II)- and SNP-induced lipid peroxidation in rat brain and liver homogenates.

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