Methods: Serum samples and clinical data of 78 subjects with acute DILI enrolled in DILIN obtained within 2 weeks of clinical onset were analyzed. Subjects were followed for 6 months or longer to determine outcome (recovery, death/liver INK 128 mw transplant). miRNA profiles in serum were compared to those from 40 healthy controls. miR-NAs were isolated from 200 μL of serum and samples were hybridized to miRNA chip containing 1733 miRNAs and 1658 probes for pre-miRNAs. Descriptive statistics were compared using the Student’s t-test or analysis of variance (ANOVA), and univariate analyses were performed to compare those who died within 6 months

vs. those who survived. ANOVA with Benjamini-Hochberg false discovery rate correction was used and an adjusted p<0.05 was considered significant. Results: The mean age of the DILI cohort was 48 years-old, 55% were female and 78% Caucasian. 55% developed hepatocellular injury click here and 22% cholestatic injury. 10 (12.8%) subjects died, 9 due to liver disease within 6 months of DILI onset. One died of non-DILI cause. Among 1733 miRNA’s analyzed 8 (122, 4532, 4484, 4463, 4270,1246, 4433, 4767) had elevated serum levels, while 3 (455-3p,

1281, 4274) decreased levels, (p<0.0001), in acute DILI cases compared to controls. 7 of the increased miRNAs were significantly correlated with ALT (p<0.01) (except 4532). miRNA-122 was increased the greatest [18-fold] [p = 10-11]. Among the 1733 miRNAs, 3 were associated with death within 6 months (miRNA−122,−

4463, −4270, P<0.05). None of the subjects with miRNA-122 greater than the median (8.31) died within 6 months. The combination of miRNA-122 serum level <7.89 and serum albumin <2.8 g/dL had sensitivity, specificity, PPV, NPV and accuracy of 100%, 81%, 38%, 100%, 83%, respectively. Conclusions: Acute DILI is associated with significant changes in a relatively small subset of serum miRNAs. The liver specific miRNA-122 combined with serum albumin levels accurately identified subjects who were likely to die within 6 months of DILI onset. If confirmed in other cohorts, serum levels of miRNA-122 and albumin, early in the course of disease, may be useful in identifying patients at greatest risk for mortality. 上海皓元医药股份有限公司 Disclosures: Mark W. Russo – Grant/Research Support: Merck; Speaking and Teaching: Gilead, Janssen, Salix, Bayer Naga P. Chalasani – Consulting: Salix, Abbvie, Lilly, Boerhinger-Ingelham, Aegerion; Grant/Research Support: Intercept, Lilly, Gilead, Cumberland, Galectin Robert J. Fontana – Consulting: GlaxoSmithKline; Grant/Research Support: Gilead, vertex, BMS, Jansen Herbert L. Bonkovsky – Advisory Committees or Review Panels: Clinuvel, Inc., Novartis Pharmaceuticals, Clinuvel, Inc., Novartis Pharmaceuticals, Clinuvel, Inc., Novartis Pharmaceuticals, Clinuvel, Inc., Novartis Pharmaceuticals; Consulting: Alnylam, Inc, Clinuvel, Inc., Novartis Pharmaceuticals, Lundbeck Pharmaceuticals, Boehringer-Ingelheim, Clinuvel, Inc.