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J: Multiple comparison procedures updated. Clin Expt Pharmacol Physiol 1998, 25:1032–1037.CrossRef Authors’ contributions JAB participated in preparation of the paper, developing the experimental design, and overseeing the experiments and performed the following experimental work: preparation Selleckchem LEE011 of C. jejuni inocula, culture of C. jejuni from mouse tissues, and scoring of culture results. JLS conducted the RFLP analysis of virulence genes. AJM conducted culture of C. jejuni from mouse tissues, DNA isolation from mouse tissues for C. jejuni-specific PCR, confirmatory PCR for C. jejuni, MLST typing of strain NW, and ELISA. VAR conducted blood and tissue harvesting from mice at necropsy. AEPJ, ELS and LSM participated in development of the C. jejuni whole Glutamate dehydrogenase ORF microarray, and AEPJ performed the hybridizations. JEW analyzed the microarray data. JRG confirmed the IL-10 genotype in the mice by PCR and performed PCR amplification
and sequencing of ORFs Cj0874c and Cj0987c. TSW performed an initial analysis of published MLST data that guided the choice of strains to be studied. LSM gained funding for the projects, participated in developing the experimental design, preparing the paper, and overseeing the experiments, and performed the following experimental work: clinical assessments of mice, blood and tissue harvesting from mice at necropsy and evaluation of hematoxylin and eosin stained tissue sections.”
“Background Trichomonads constitute a group of protists belonging to the phylum Parabasala that are mostly parasitic or commensal flagellates inhabiting oxygen-poor environments [1]. Trichomonas vaginalis is responsible for the number one, non-viral sexually transmitted disease (STD) with ~9 million new cases of women with trichomonosis in the US alone, and 250–350 million worldwide [2–5]. This STD causes serious adverse health outcomes in women, including adverse pregnancy outcomes, cervical neoplasia, atypical pelvic inflammatory disease, and infertility [6–8]. Men with trichomonosis may have non-gonococcal urethritis, prostatitis, epydidymitis, and infertility [8, 9].