In previous experiments, it has already been shown in vitro that L. gasseri K7 survived

in an acidic environment and with 0.3% bile salts [10]. These findings make the strain interesting as a possible probiotic. In this study, a single bioreactor system based on the work of Sumeri et al. [9] was used to evaluate the survival of Lactobacillus gasseri K7 and eight Bifidobacterium strains from our collection. We were able to compare the results for L. gasseri K7 with a study performed in piglets [14] which allowed the assessment of a correlation between the in-vitro study with results from in-vivo experiments. The retention times and pH used in this study were based on data from the literature. Several methods exist for measuring the pH in the intestine [15]. Table 1 shows the pH values

in the different parts of the intestine as #click here randurls[1|1|,|CHEM1|]# measured by the Heidelberg capsule [16, 17]. Retention times can be calculated either by using marker substances (chemical) or by radio telemetry capsules such as the Heidelberg capsule [18]. However, capsules usually have longer retention times than chemical markers. Table 2 lists some of the retention times found in the literature [4, 5, 19–24]. Table 1 pH values in the human intestinal tract, measured with the Heidelberg capsule. Epacadostat order   Stomach Duodenum Jejunum Ileum       proximal medial Distal pH 1.4** 6.22* 6.4** 7.1** 7.4** * Fallingborg et al. 1994 [16] ** Fallingborg et al. 1998 [17] Table 2 Retention times in the small intestine cited in literature. Retention time Source Remarks 1–4 h Huang and Adams 2004 [21]   4.25 h Van Den Driessche et al. 2000 [24] Stomach and small intestine 4 h Mojaverian 1996 [22]   6 h Picot and Lacroix 2004 [4] Selected maximum time of the simulation 7.5 h Fallingborg et al. 1990 [20] Children 8 h Fallingborg

et al. 1989 [19]   8 h Alander et al. 1998 [5] Simulation in the SHIME Reactor 6–10 h Thews et al. 1991 [23]   Based on Dipeptidyl peptidase the data found in the literature and the work by Sumeri et al. [9] the fermentation process was set up as described in Material and Methods and is shown in Figure 1. Figure 1 Parameters of the stomach-intestinal passage simulation over 7 h. Results Acid resistance screening The aim of an initial series of tests was to obtain an overview of the acid resistance of eight bifidobacteria strains. Figures 2, 3 and 4 show the survival of these strains using contour plots made with Sigmaplot. Bifidobacterium dentium (Figure 3) showed the least acid resistance. Between pH 4.0 and pH 2.0 there was no difference in survival and the concentration of cells dropped by more than 7 log within 40 minutes. Bifidobacterium animalis subsp. lactis was more resistant up to 40 min at pH 2.0, but then decreased by about 3 log when incubated for 120 minutes (Figure 4). At a pH between 2.5 and 3.0 the decrease was less than 1 log after 120 minutes.