However, the promoters, which do not damage DNA directly, can facilitate tumor development from initiated cells. Now, more and more chemicals have Crenigacestat mw been identified as tumor promoters in experimental animals and in cell transformation models, and their molecular mechanisms have been undoubtedly elucidated [8]. Two of the most frequently used chemicals are MNNG and PMA. For example, BALB/c-3T3-cell was successfully transformed by MNNG and PMA treatment [9]. As a consequence result, transformed foci were the final outcome of transforming cells in a malignant state. This kind of transformation assay can detect both initiating and promoting activities, which might be a screening tool for detection

of not only tumor initiators but

also tumor promoters such as non-genotoxic GSK2879552 carcinogens. The process of adenoma growth and transformation was accompanied by cumulative mutations in genetic pathways that confer a growth advantage of colon cancer. These pathways included cell cycle controlling, cell signaling pathway, cell apoptosis and adhesion [10]. So the major challenge is to identify the molecular signatures that indicate increased likelihood for colon cancer progression. Most of importantly, it has been reported that microRNA (miRNAs) was involved in the development of caner [11, 12]. Characteristic patterns of miRNAs expression are precisely regulated. Deviations from normal pattern of expression may play a role in diseases, such as in tumorigenesis and progress. Indeed, altered miRNAs expression has been reported in many types of cancer cells, although the Compound Library nmr functional significance of these changes has yet to be fully addressed [13, 14]. As colon caner concerned, aberrantly expressed or mutation of individual miRNAs were reported [15–17]. For example, miR-143 and miR-145 consistently display reduced steady-state Quinapyramine levels of the mature miRNAs at the adenomatous and cancer stages of colorectal neoplasia, by comparative analyzing of human samples. Furthermore, miR-143 and miR-145 would be potentially useful as diagnostic and therapeutic tools for colon cancer and other types

of cancer [18, 19]. With the accumulating evidences in the literature that new genes found to be implicated in colon cancer, the detailed molecular mechanism underlying the development and progress of colon cancer remains unknown. To find out the genes associated with cancer biological pathways involved in transformation and tumorigenesis, we transformed normal IEC-6 cells to cancer cells by treatment with cancerogenic agent of MNNG and PMA. IEC-6 cell line was derived from normal rat intestinal epithelia [20]. We transformed IEC-6 cells, and identified the altered gene expression by rat Oligo GEArray microarray of the six biological pathways involved in transformation and tumorigenesis. At the same, we indentified the altered miRNAs of transformed IEC-6 cells by array hybridization.