005, P TrxB, HCl = 0.009, P Cj0706, Ac = 0.016, P MogA, HCl, Ac < 0.03). Volume% of bacterioferritin (Dps) during HCl stress was higher compared with the control, but probably due to the variation of the control this difference was not significant (P 11168, Dps, HCl = 0.061). For the acid-robust strain 305, Dps, p19, MogA and TrxB were significantly induced (P Dps, HCl = 0.0028, P p19, HCl = 0.0008, P MogA, HCl = 0.018, P TrxB, HCl = 0.017). Fewer proteins were induced in the acid-sensitive

strain 327, which was also reduced during the acid stress (Figure  2B). Only induction of Cj0706 and MogA was observed during HCl acid stress (P Cj0706, HCl = 0.0037, P MogA, HCl = 0.04). In the case of NCTC 11168 and 305, the two proteins alkyl hydroperoxide reductase (AhpC) and superoxide dismutase (SodB) had higher% ��-Nicotinamide cost volume intensity PF-01367338 solubility dmso than for the control indicating induction; however the differences were not significant. A reference profile of proteins separated by 2D-electrophoresis for C. jejuni 305 exposed to HCl stress (pH 5.2) NCT-501 manufacturer is shown in Figure  3. Table 3 Induced proteins (% volume intensity) during HCl (pH 5.2) and acetic acid (pH 5.7) exposure in C. jejuni NCTC 11168, C. jejuni 305 and C. jejuni 327 at 37°C in chemically defined broth    

    Campylobacter jejuni strains3 Protein/(NCBInr 1 ) Mw (kDa) Score 2   NCTC 11168 305 327 Dps (NP282665) 17.4 222 Vol% p19 (CAA73983) 17.0 255 Vol% AhpC (NP281525) 22.0 668 Vol% SodB (NP281379) 25.0 241 Vol% TrxB (NP281357) 33.5 204 Vol% Cj0706 (NP281878) 28.0 431 Vol% MogA (YP_178829) 20.3 318 Vol%   C HCl Ac C HCl Ac C HCl Ac Dps: Bacterioferritin,

p19: 19 kDa periplasmic protein, AhpC: Alkyl hydroperoxide reductase, SodB: Superoxide dismutase Clomifene (Fe), TrxB: Thioredoxin-disulfide reductase, Cj0706: hypothetical protein, MogA: Molybdenum cofactor biosynthesis protein. Columns: light grey: control (C), dark grey: HCl stressed cells (HCl), white: Acetic acid stressed cells (Ac). 1 Identification was based on Mascot MS/MS Ion Search using sequence data from the database NCBInr. 2 Mowse Score (Score). 3 The intensity of the induced proteins was estimated by Image MasterTM 2D Platinum and % volume intensity was calculated. The intensity of the protein spots was analyzed using the Image MasterTM 2D Platinum (version 5.0, Amersham Biosciences, Melanie). Three biological independent replicates was performed and % volume intensity was calculated as: % volume intensity control (protein x) = volume intensity /(volume intensity control + volume intensity HCl + volume intensity acetic acid). Figure 3 Reference map of proteins from C. jejuni 305 separated by 2D-gel-electrophoresis. The strain was grown in modified chemically defined broth modified (CDB) containing 0.01 mM methionine at 37°C to late exponential phase and until the cell level was 1 × 108 CFU/ml.