​(Fig.3).3). PKCγ

immunolabeling exhibited a similar pattern of distribution in the sham (Fig. ​(Fig.3A)3A) and 6-OHDA-lesioned (allodynic) rats (Fig. ​(Fig.3B).3B). However, staining intensity had increased bilaterally in the 6-OHDA-lesioned rats in comparison to the shams. Consistent with previous studies (Clifford et al. 1998; Okada-Ogawa et al. 2009), PKCγ immunostaining was mainly observed in lamina IIi of the MDH Inhibitors,research,lifescience,medical at both the ipsilateral and contralateral sides. Some scattered PKCγ-positive cells were also observed within laminae I and outer II (IIo). Careful inspection of PKCγ immunolabeling under high magnification confirmed these observations. Interestingly, we noticed a marked increase in both the intensity of staining (Fig. ​(Fig.3B)3B) and the number (Fig. ​(Fig.3C,3C, P < 0.001) of PKCγ-positive cells

in the MDH lamina III in allodynic animals. The intensity of PKCγ staining within lamina IIi was significantly higher in the 6-OHDA-lesioned animal (Fig. ​(Fig.3D)3D) when compared to Inhibitors,research,lifescience,medical the shams. Figure 3 PKCγ staining in sham (A) and allodynic MDH (B). Very intense PKCγ labeling can be seen in the 6-OHDA-lesioned MDH (B) compared to that of the shams (A). This labeling is observed in the cells of lamina IIi and in scattered Inhibitors,research,lifescience,medical cells within … Mild nonnoxious stimulus induced pERK1/2 expression in MDH laminae In the 6-OHDA-lesioned rats that received selleck compound tactile stimuli (Fig. ​(Fig.4),4), most of the pERK1/2-positive cells were observed in the superficial laminae I and II, in particular in the ipsilateral side receiving the stimulus (Fig. ​(Fig.4A).4A). The superficial lamina II contained the largest number of pERK1/2 Inhibitors,research,lifescience,medical immunopositive neurons, which had increased considerably on the ipsilateral side in comparison to the contralateral side (Fig. ​(Fig.4B).4B). Only a very small number of pERK1/2-positive cells were observed in the sham animals (Fig. ​(Fig.4C).4C). Generally, these cells were scattered and located in the whole nucleus and were not restricted

to the MDH Inhibitors,research,lifescience,medical region that had received a mild nonnoxious stimulation. The number of pERK1/2-positive cells in the ipsilateral superficial lamina of the MDH I of the allodynic rats was significantly higher in comparison to the contralateral or sham rats (Fig. ​(Fig.4D).4D). For laminae III–V, there was no difference between the allodynic rats in comparison to the sham rats regarding the number of Phosphatidylinositol diacylglycerol-lyase pERK1/2-positive cells, ipsilateral or contralateral to the operated IoN. The general pattern of pERK1/2 expression was similar to that obtained previously (Okada-Ogawa et al. 2009). Figure 4 Mild nonnoxious stimulus induced pERK1/2 expression in MDH laminae. In 6-OHDA-lesioned rats (A–B), gentle tactile stimuli induce the expression of pERK1/2 (arrow) in cells located in the ipsilateral superficial laminae I and II. pERK1/2 expression …