From several independent EPZ5676 price measurements, means and standard deviations were calculated. Data are shown as mean ± SD from at least three separate

experiments. Testing for significant differences between means was carried out using one-way ANOVA and Dunnett’s Multiple Comparison test at a probability of error of 5% (*), 1% (**) and 0.1% (***). Two silica-based NPs were investigated: 1. Sicastar Red (amorphous silica; primary particles ca. 30 nm in diameter) and 2. AmOrSil [(poly(organosiloxane) with a shell of poly(ethylene oxide), PEO, to ensure particle solubility in water; primary particles ca. 60 nm in diameter)]. Fig. 1A depicts the viability (MTS assay) and membrane integrity (LDH assay) of the lung epithelial cell line H441 and the microvascular endothelial

cell line ISO-HAS-1 cultured in conventional monocultures (MC) after exposure to Sicastar Red and AmOrSil for 4 h in serum-free medium. According to MTS, H441 showed a significantly reduced viability at high concentrations of Sicastar Red (100 μg/ml: 14 ± 12%; 300 μg/ml: 60 ± 12% compared to untreated control uc), whereas AmOrSil did not have any effect (e.g. 300 μg/ml: 109 ± 12% compared to uc). Similar observations have been made for the microvascular endothelial cell line ISO-HAS-1 with Sicastar Red (300 μg/ml: 36 ± 18% and 100 μg/ml: 34 ± 4% of uc) as well as AmOrSil (300 μg/ml 111 ± 15% of uc). Sicastar Red did not cause a significant decrease in the mitochondrial activity at 60 μg/ml for both cell types (H441: 98 ± 15%

and ISO-HAS-1: 99 ± 12% of uc). With respect to GW3965 viability, similar effects were obtained for the membrane integrity after NP exposure. H441 showed a significant release of LDH after 4 h exposure to Sicastar Montelukast Sodium Red (300 μg/ml: 90 ± 7.5%, 100 μg/ml: 70 ± 13.6%, 60 μg/ml: 46 ± 22% of lysis control lc), whereas 6 μg/ml Sicastar Red did not show any toxic effects (14.2 ± 12% of lc). Similar to H441, ISO-HAS-1 also displayed a high LDH release at high concentrations (300 μg/ml: 77 ± 7.5%, 100 μg/ml: 57 ± 18% of lc) but not at 60 μg/ml (12 ± 5% of lc). AmOrSil did not cause a change in membrane integrity even at high concentrations of 300 μg/ml in H441 or ISO-HAS-1 (H441: 13 ± 11% and ISO-HAS-1: 4 ± 2.8% of lc). According to Fig. 1B, LDH release into the apical compartment (H441) of the coculture (CC) was firstly detected at a concentration of 100 μg/ml Sicastar Red (30 ± 5.6% of lysis control, 2-fold of untreated control uc), but to a lower extent as observed for the H441 in MC (57 ± 18% of lc). The LDH release of the H441 in CC further increased with increasing concentrations (300 μg/ml: 49.3 ± 12.4% of lc), which is also lower compared to the MC (90 ± 7.5% of lc). A concentration of 60 μg did not yield higher LDH levels (10.4 ± 2.5% of lc) on the contrary to the MC (46 ± 22% of lc).